Dnmt3a^em5Mago
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dnmt3a^em5Mago
• Name: DNA methyltransferase 3A; endonuclease-mediated mutation 1, Margaret A Goodell
• MGI ID: MGI:7414394
• Synonyms: Dnmt3a1 deltaN
• Gene: Dnmt3a Location: Chr12:3856007-3964443 bp, + strand Genetic Position: Chr12, 1.99 cM, cytoband A2-A3
• Alliance: Dnmt3a^em5Mago page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Modified isoform(s))
• Mutation: Intragenic deletion
• Mutation details: CRISRP/Cas9 technology deleted exons 3-6, leading to an in-frame deletion of amino acids 22-209 which constitutes 86% of the N terminus. Isoform 1 (Dnmt3a1) thus lacks most of the N terminus but is transcribed normally. Expression of the truncated protein was confirmed in the brain. (J:331942)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnmt3a Mutation: 140 strains or lines available

References

• Original: J:331942 Gu T, et al., The disordered N-terminal domain of DNMT3A recognizes H2AK119ub and is required for postnatal development. Nat Genet. 2022 May;54(5):625-636
• All: 1 reference(s)