Dnmt3a^em4Mago
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dnmt3a^em4Mago
• Name: DNA methyltransferase 3A; endonuclease-mediated mutation 4, Margaret A Goodell
• MGI ID: MGI:7414393
• Synonyms: Dnmt3a1^r4
• Gene: Dnmt3a Location: Chr12:3856007-3964443 bp, + strand Genetic Position: Chr12, 1.99 cM, cytoband A2-A3
• Alliance: Dnmt3a^em4Mago page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Null/knockout)
• Mutation: Insertion
• Mutation details: Exon 4 was replaced by a reversed version of exon 4 that is flanked by lox71 and lox66 sites via CRISPR/Cas9 technology. Western blot analysis confirmed absence of protein expression (both isoform 1 and 2). The correct orientation of exon 4, and thus expression of isoform 1, is restored after cre-mediated recombination. (J:331942)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnmt3a Mutation: 140 strains or lines available

References

• Original: J:331942 Gu T, et al., The disordered N-terminal domain of DNMT3A recognizes H2AK119ub and is required for postnatal development. Nat Genet. 2022 May;54(5):625-636
• All: 1 reference(s)