Rest^{tm2c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rest^{tm2c(EUCOMM)Wtsi}
• Name: RE1-silencing transcription factor; targeted mutation 2c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7414372
• Synonyms: Rest^f
• Gene: Rest Location: Chr5:77413338-77434279 bp, + strand Genetic Position: Chr5, 41.61 cM
• Alliance: Rest^{tm2c(EUCOMM)Wtsi} page
• IMPC: Rest gene page

Mutation origin

• Mutant Cell Line: EPD0156_1_B12
• Germline Transmission: Earliest citation of germline transmission: J:330714
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 77415210 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 77416960. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:330714)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rest Mutation: 92 strains or lines available

References

• Original: J:330714 Cloud AS, et al., Loss of the repressor REST affects progesterone receptor function and promotes uterine leiomyoma pathogenesis. Proc Natl Acad Sci U S A. 2022 Nov;119(44):e2205524119
• All: 1 reference(s)