Tg(RP23-333I12)#Jeng
Transgene Detail

Summary

• Symbol: Tg(RP23-333I12)#Jeng
• Name: transgene insertion, James Douglas Engel
• MGI ID: MGI:7414207
• Synonyms: 333I12/G2lacZ
• Transgene: Tg(RP23-333I12)#Jeng Location: unknown
• Alliance: Tg(RP23-333I12)#Jeng page

Transgene origin

• Strain of Origin: Not Applicable

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The transgene insert contains the following elements: an FRT site flanked neomycin resistance gene cassette, Gata2 upstream regulatory sequence (from ~4 kb upstream of exon 1S to ATG translation start in exon 2; contains upstream alternative promoter, 1st exon, and intron 1S and downstream alternative promoter, 1st exon and intron 1G, and 5' UTR part of exon 2), the lacZ reporter gene, and BAC RP23-333I12 (which contains the 5' end of Eefsec with Gata2 enhancers Rr341 and Rr342 in its introns). The neo cassette was removed through subsequent flp-mediated recombination. The reporter gene is expressed in urogenital mesenchymal (but not epithelial) tissue, and digits, boundary cap cells, otic vesicle, hind- and midbrain, nose and other tissues. (J:95123)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:95123 Khandekar M, et al., Multiple, distant Gata2 enhancers specify temporally and tissue-specific patterning in the developing urogenital system. Mol Cell Biol. 2004 Dec;24(23):10263-76
• All: 1 reference(s)