Bmp4^tm2Jlch
Targeted Allele Detail

Summary

• Symbol: Bmp4^tm2Jlch
• Name: bone morphogenetic protein 4; targeted mutation 2, Jan L Christian
• MGI ID: MGI:7413956
• Synonyms: Bmp4^S2KHAMycNeo
• Gene: Bmp4 Location: Chr14:46620982-46628126 bp, - strand Genetic Position: Chr14, 23.95 cM
• Alliance: Bmp4^tm2Jlch page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:213855
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Epitope tag)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The targeting vector includes a) a sequence encoding an in-frame HA epitope tag within the prodomain following amino acid 61, b) sequence encoding an in-frame myc tag within the mature domain, four amino acids downstream of the S1 cleavage site, c) three point mutations in exon 4 that introduce a serine to lysine amino acid change at the S2 cleavage site (RISR-RIKR) and a new BglII site, and d) a neomycin selectable marker flanked by loxP sites upstream of exon 4. Wheareas, wild-type BMP4 is sequentially cleaved at the S1 and then the S2 site, the S2K mutation allows for simultaneous cleavage of both sites. (J:213855)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bmp4 Mutation: 21 strains or lines available

References

• Original: J:213855 Tilak A, et al., Simultaneous rather than ordered cleavage of two sites within the BMP4 prodomain leads to loss of ligand in mice. Development. 2014 Aug;141(15):3062-71
• All: 1 reference(s)