Hdac8^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Hdac8^{tm1d(EUCOMM)Wtsi}
• Name: histone deacetylase 8; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7408100
• Synonyms: Hdac8^(KO)
• Gene: Hdac8 Location: ChrX:101328245-101548965 bp, - strand Genetic Position: ChrX, 45.28 cM, cytoband C3
• Alliance: Hdac8^{tm1d(EUCOMM)Wtsi} page
• IMPC: Hdac8 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:274458
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_gt2 cassette was inserted at position 101543196 of Chromosome X upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 101543998. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:274458)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hdac8 Mutation: 12 strains or lines available

References

• Original: J:274458 Qi J, et al., HDAC8 Inhibition Specifically Targets Inv(16) Acute Myeloid Leukemic Stem Cells by Restoring p53 Acetylation. Cell Stem Cell. 2015 Nov 5;17(5):597-610
• All: 1 reference(s)