Hdac8^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Hdac8^{tm1c(EUCOMM)Wtsi}
• Name: histone deacetylase 8; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7408099
• Synonyms: Hdac8^floxed
• Gene: Hdac8 Location: ChrX:101328245-101548965 bp, - strand Genetic Position: ChrX, 45.28 cM, cytoband C3
• Alliance: Hdac8^{tm1c(EUCOMM)Wtsi} page
• IMPC: Hdac8 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:274458
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt2 cassette was inserted at position 101543196 of Chromosome X upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 101543998. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:274458)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hdac8 Mutation: 12 strains or lines available

References

• Original: J:274458 Qi J, et al., HDAC8 Inhibition Specifically Targets Inv(16) Acute Myeloid Leukemic Stem Cells by Restoring p53 Acetylation. Cell Stem Cell. 2015 Nov 5;17(5):597-610
• All: 5 reference(s)