Rasal1^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rasal1^{tm1b(KOMP)Wtsi}
• Name: RAS protein activator like 1 (GAP1 like); targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:7408093
• Gene: Rasal1 Location: Chr5:120786877-120817662 bp, + strand Genetic Position: Chr5, 60.63 cM
• Alliance: Rasal1^{tm1b(KOMP)Wtsi} page
• IMPC: Rasal1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:331889
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 120792385 of Chromosome 5 upstream of critical exons 3 and 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exons 3 and 4 at position 120793472. Exons 3 and 4 are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:331889)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rasal1 Mutation: 52 strains or lines available

References

• Original: J:331889 Xu R, et al., A semaphorin-plexin-Rasal1 signaling pathway inhibits gastrin expression and protects against peptic ulcers. Sci Transl Med. 2022 Jul 20;14(654):eabf1922
• All: 1 reference(s)