Nxn^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Nxn^{tm1c(EUCOMM)Wtsi}
• Name: nucleoredoxin; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7408062
• Synonyms: Nxn^fl
• Gene: Nxn Location: Chr11:76148052-76289967 bp, - strand Genetic Position: Chr11, 45.92 cM
• Alliance: Nxn^{tm1c(EUCOMM)Wtsi} page
• IMPC: Nxn gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:331887
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt0 cassette was inserted at position 76168930 of Chromosome 11 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 2 at position 76169881. The critical exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon 2. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:331887)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nxn Mutation: 498 strains or lines available

References

• Original: J:331887 Valek L, et al., Cold avoidance and heat pain hypersensitivity in neuronal nucleoredoxin knockout mice. Free Radic Biol Med. 2022 Sep 17;192:84-97
• All: 2 reference(s)