Hsp90ab1^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Hsp90ab1^{tm1d(EUCOMM)Wtsi}
• Name: heat shock protein 90 alpha (cytosolic), class B member 1; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7408057
• Synonyms: HSP90beta KO
• Gene: Hsp90ab1 Location: Chr17:45878704-45884187 bp, - strand Genetic Position: Chr17, 22.59 cM
• Alliance: Hsp90ab1^{tm1d(EUCOMM)Wtsi} page
• IMPC: Hsp90ab1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:331876
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 45880977 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 45883284. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:331876)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hsp90ab1 Mutation: 43 strains or lines available

References

• Original: J:331876 Bhattacharya K, et al., Translational reprogramming in response to accumulating stressors ensures critical threshold levels of Hsp90 for mammalian life. Nat Commun. 2022 Oct 21;13(1):6271
• All: 1 reference(s)