Hsp90ab1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Hsp90ab1^{tm1c(EUCOMM)Wtsi}
• Name: heat shock protein 90 alpha (cytosolic), class B member 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7408054
• Gene: Hsp90ab1 Location: Chr17:45878704-45884187 bp, - strand Genetic Position: Chr17, 22.59 cM
• Alliance: Hsp90ab1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Hsp90ab1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:331876
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 45880977 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 45883284. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:331876)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hsp90ab1 Mutation: 46 strains or lines available

References

• Original: J:331876 Bhattacharya K, et al., Translational reprogramming in response to accumulating stressors ensures critical threshold levels of Hsp90 for mammalian life. Nat Commun. 2022 Oct 21;13(1):6271
• All: 1 reference(s)