Neb^tm5.1Hgra
Targeted Allele Detail

Summary

• Symbol: Neb^tm5.1Hgra
• Name: nebulin; targeted mutation 5.1, Henk Granzier
• MGI ID: MGI:7407200
• Synonyms: Neb^Dupl(S9-11)
• Gene: Neb Location: Chr2:52026652-52228810 bp, - strand Genetic Position: Chr2, 29.98 cM
• Alliance: Neb^tm5.1Hgra page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:331852
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Intragenic deletion
• Mutation details: A targeting vector was designed using the Osdd vector (providing TK for negative selection) with a 3.4kb left- and 3.5kb right- arm of homology. This vector replaces 1347bp (chr2:52,250,794-52,252,140) including exon 64 with a 2.8kb synthetic cDNA fragment with exon 64+exons 53-64 (and flanking intronic sequence) followed by a neoR positive selection cassette. Flp-mediated recombination removed the neoR casette. (J:331852)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Neb Mutation: 399 strains or lines available

References

• Original: J:331852 Kiss B, et al., Nebulin and Lmod2 are critical for specifying thin-filament length in skeletal muscle. Sci Adv. 2020 Nov;6(46)
• All: 1 reference(s)