Kcnh2^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Kcnh2^{tm1c(EUCOMM)Wtsi}
• Name: potassium voltage-gated channel, subfamily H (eag-related), member 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7407143
• Synonyms: Kcnh2^flox, Kcnh2^loxp
• Gene: Kcnh2 Location: Chr5:24524587-24556602 bp, - strand Genetic Position: Chr5, 10.94 cM
• Alliance: Kcnh2^{tm1c(EUCOMM)Wtsi} page
• IMPC: Kcnh2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:303317
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 24529095 of Chromosome 5 upstream of the critical exons 6-9 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 6-9 at position 24532275. The critical exons 6-9 are thus flanked by loxP sites. Flp-mediated recombination deleted the lacZ and neomycin resistance gene generating a "conditional ready" exon 6-9 floxed allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:303317)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kcnh2 Mutation: 45 strains or lines available

References

• Original: J:303317 Dierich M, et al., Optimized Tuning of Auditory Inner Hair Cells to Encode Complex Sound through Synergistic Activity of Six Independent K(+) Current Entities. Cell Rep. 2020 Jul 7;32(1):107869
• All: 2 reference(s)