Cd3e^{tm1.1(CD3E)Bms}
Targeted Allele Detail

Summary

• Symbol: Cd3e^{tm1.1(CD3E)Bms}
• Name: CD3 antigen, epsilon polypeptide; targeted mutation 1.1, Bristol Myers Squibb
• MGI ID: MGI:7398576
• Synonyms: hCD3E-epi
• Gene: Cd3e Location: Chr9:44910038-44920925 bp, - strand Genetic Position: Chr9, 24.84 cM
• Alliance: Cd3e^{tm1.1(CD3E)Bms} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:331729
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutation: Insertion
• Mutation details: Exon 4 was replaced with human exon 4 and 5' sequences from human exon 5 were added to the 5' end of mouse exon 5. This replaces mouse amino acid sequence DAENI with its human counterpart GNEEMGGITQT, which encodes a common epitope recognized by anti-human CD3E antibodies. A loxP site flanked neomycin resistance gene cassette was inserted in intron 4 and was subsequently removed via Cre-mediated recombination. (J:331729)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd3e Mutation: 51 strains or lines available

References

• Original: J:331729 Zorn JA, et al., Humanization of a strategic CD3 epitope enables evaluation of clinical T-cell engagers in a fully immunocompetent in vivo model. Sci Rep. 2022 Mar 3;12(1):3530
• All: 1 reference(s)