Mir22^tm1.1Mpo
Targeted Allele Detail

Summary

• Symbol: Mir22^tm1.1Mpo
• Name: microRNA 22; targeted mutation 1.1, Matti Poutanen
• MGI ID: MGI:7398205
• Gene: Mir22 Location: Chr11:75354542-75354636 bp, + strand Genetic Position: Chr11, 45.91 cM
• Alliance: Mir22^tm1.1Mpo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:331713
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: BAC clone CH29-40010 containing the mouse Mir22 gene was manipulated through Red/ET recombination technology. A loxP was inserted in the 5 flanking region of exon 1 and a second loxP site was introduced in the 3 flanking region of exon 1 with a PGK-FRT-Neo-FRT-loxP cassette. Targeted ES cells were re-electroporated with a flp-recombinase expressing plasmid to remove the neomycin cassette leaving exon 1 floxed. Exon 1 was deleted via cre-mediated recombination in the germline. RT-qPCR confirmed undetectable levels of mature miR-22-3p in the liver, epididymal white adipose tissue and gastrocnemius tissue. (J:331713)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir22 Mutation: 7 strains or lines available

References

• Original: J:331713 Gjorgjieva M, et al., Genetic Ablation of MiR-22 Fosters Diet-Induced Obesity and NAFLD Development. J Pers Med. 2020 Oct 14;10(4):170
• All: 2 reference(s)