Ythdc1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ythdc1^{tm1c(EUCOMM)Wtsi}
• Name: YTH domain containing 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7388373
• Synonyms: Ythdc1^flox
• Gene: Ythdc1 Location: Chr5:86952080-86984518 bp, + strand Genetic Position: Chr5, 43.56 cM
• Alliance: Ythdc1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Ythdc1 gene page

Mutation origin

• Mutant Cell Line: EPD0113_2_A08
• Germline Transmission: Earliest citation of germline transmission: J:331492
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 86967482 of Chromosome 5 upstream of the critical exons 5-7 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exons at position 86969487. The critical exons 5-7 are thus flanked by loxP sites. Flp-mediated recombination removed the lacZ/neomycine leaving exons 5-7 floxed. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:331492)

Expression

In Mice Carrying this Mutation:

3 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ythdc1 Mutation: 53 strains or lines available

References

• Original: J:331492 Li X, et al., Downregulation of the m(6)A reader protein YTHDC1 leads to islet beta-cell failure and diabetes. Metabolism. 2022 Oct 24;138:155339
• All: 6 reference(s)