Ep300^tm1.1Kahn
Targeted Allele Detail

Summary

• Symbol: Ep300^tm1.1Kahn
• Name: E1A binding protein p300; targeted mutation 1.1, Michael Kahn
• MGI ID: MGI:7388161
• Synonyms: p300 S89A
• Gene: Ep300 Location: Chr15:81470329-81536273 bp, + strand Genetic Position: Chr15, 38.16 cM
• Alliance: Ep300^tm1.1Kahn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:331371
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: The targeting vector contains, from 5' to 3', a loxP site, a lox2272 site, a mutated exon 2 of the gene (in reverse orientation), a second loxP site, an FRT-flanked neomycin resistance cassette, and endogenous exon 2 followed by a second lox2272 site. The mutant exon contains a AGC to GCC mutation resulting in a serine to alanine change (S89A) which removes a highly conserved phosphorylation site at S89. The two pairs of heterospecific lox sites are inward-facing. Flp-mediated recombination removed the neomycin cassette. Cre-mediated recombination inverted the S89A mutated fragment to the correct orientation. (J:331371)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ep300 Mutation: 98 strains or lines available

References

• Original: J:331371 Lai KKY, et al., p300 Serine 89: A Critical Signaling Integrator and Its Effects on Intestinal Homeostasis and Repair. Cancers (Basel). 2021 Mar 14;13(6)
• All: 1 reference(s)