Vps45^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Vps45^{tm1c(EUCOMM)Hmgu}
• Name: vacuolar protein sorting 45; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7387114
• Synonyms: Vps45^fl
• Gene: Vps45 Location: Chr3:95907144-95965778 bp, - strand Genetic Position: Chr3, 41.65 cM
• Alliance: Vps45^{tm1c(EUCOMM)Hmgu} page
• IMPC: Vps45 gene page

Mutation origin

• Mutant Cell Line: HEPD0764_7_D04
• Germline Transmission: Earliest citation of germline transmission: J:331313
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 95955412 of Chromosome 3 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 95956717. The critical exon 4 is thus flanked by loxP sites. The neomycin resistance gene was removed via flp-mediated recombination generating a conditional ready (floxed) allele. (J:331313)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vps45 Mutation: 28 strains or lines available

References

• Original: J:331313 Frey L, et al., Mammalian VPS45 orchestrates trafficking through the endosomal system. Blood. 2021 Apr 8;137(14):1932-1944
• All: 1 reference(s)