Rr149356^tm2.1Fwa
Targeted Allele Detail

Summary

• Symbol: Rr149356^tm2.1Fwa
• Name: regulatory region 149356; targeted mutation 2.1, Frederick W Alt
• MGI ID: MGI:7386933
• Synonyms: EbetaRdelta
• Gene: Rr149356 Location: Chr6:41526801-41532817 bp Genetic Position: Chr6, Syntenic
• Alliance: Rr149356^tm2.1Fwa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:34312
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Modified regulatory region)
• Mutations: Insertion, Intergenic deletion
• Mutation details: A HpaI-NcoI restriction fragment containing the core Ebeta Tcrb enhancer was replaced with a 700 bp XbaI-EcoRI fragment containing Igh enhancer iEmu (Rr150), and a loxP site flanked neomycin resistance gene cassette. The neo cassette was removed through subsequent cre-mediated recombination. (J:34312)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr149356 Mutation: 0 strains or lines available

References

• Original: J:34312 Bories JC, et al., Gene-targeted deletion and replacement mutations of the T-cell receptor beta-chain enhancer: the role of enhancer elements in controlling V(D)J recombination accessibility. Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):7871-6
• All: 1 reference(s)