Calr^tm2.1Ics
Targeted Allele Detail

Summary

• Symbol: Calr^tm2.1Ics
• Name: calreticulin; targeted mutation 2.1, Mouse Clinical Institute
• MGI ID: MGI:7384568
• Synonyms: CALR del52
• Gene: Calr Location: Chr8:85568717-85573560 bp, - strand Genetic Position: Chr8, 41.21 cM
• Alliance: Calr^tm2.1Ics page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:300217
• Parent Cell Line: S3 (ES Cell)
• Strain of Origin: C57BL/6NCrl

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutations: Insertion, Intragenic deletion
• Mutation details: CRISPR-targeting inserted a loxP site, a fusion of exon 8 and 9, a stop site and triple polyadenylation sequence, an FRT-flanked neomycin resistance cassette, a loxP site, wild-type exon 8, and a mutated exon 9 with the human mutated C-terminal tail containing a 52 bp deletion. Flp-mediated recombination removed the selection cassette. (J:300217)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Calr Mutation: 14 strains or lines available

References

• Original: J:300217 Benlabiod C, et al., Calreticulin del52 and ins5 knock-in mice recapitulate different myeloproliferative phenotypes observed in patients with MPN. Nat Commun. 2020 Sep 28;11(1):4886
• All: 1 reference(s)