Hprt1^{tm2(Myh6-GAL4)Vmc}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm2(Myh6-GAL4)Vmc}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 2, Vincent M Christoffels
• MGI ID: MGI:7382857
• Synonyms: HprtalphaMhc-Gal4
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm2(Myh6-GAL4)Vmc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:330678
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Hprt1^{tm2(Myh6-GAL4)Vmc} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  HPRT1 (3251)

  Hprt1

  (MGI:96217)

  human HPRT1 promoter and exon 1

• Mutation details: The following sequences were inserted in place of the endogenous promoter, exons 1-2 and 5' end of intron 2, which are lacking in the ES cells used: the mouse Myh6 promoter, the yeast GAL4 gene, human GH poly(A) signal sequence, human HPRT promoter and exon 1, and mouse Hprt intron 1, exon 2 and 5' part of intron 2. In this allele the Myh6 promoter drives the expression of GAL4 and the human HPRT promoter drives the expression of the restored (but now human-mouse hybrid) Hprt gene. (J:330678)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1280 strains or lines available

References

• Original: J:330678 Habets PE, et al., Cardiac expression of Gal4 causes cardiomyopathy in a dose-dependent manner. J Muscle Res Cell Motil. 2003;24(2-3):205-9
• All: 1 reference(s)