Hprt1^{tm1(Nppa-lacZ)Vmc}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm1(Nppa-lacZ)Vmc}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, Vincent M Christoffels
• MGI ID: MGI:7382844
• Synonyms: Hprt-0.7rLacZ
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm1(Nppa-lacZ)Vmc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:278530
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The following sequences were inserted in place of the endogenous promoter, exons 1-2 and 5' end of intron 2, which are lacking in the ES cells used: in antisense direction: the 638/+70 bp rat Nppa regulatory region sequence, containing the promoter and silencer and enhancer sequences, a beta-globin/IgG chimeric intron, nls sequence, the lacZ beta-galactosidase gene and bovine GH poly(A) signal sequence; in sense direction: human HPRT promoter and exon 1, and mouse Hprt intron 1, exon 2 and 5' part of intron 2. In this allele the rat Nppa regulatory region drives the expression of lacZ and the human HPRT promoter drives the expression of the restored (but now human-mouse hybrid) Hprt gene. (J:278530)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1280 strains or lines available

References

• Original: J:278530 Horsthuis T, et al., Distinct regulation of developmental and heart disease-induced atrial natriuretic factor expression by two separate distal sequences. Circ Res. 2008 Apr 11;102(7):849-59
• All: 1 reference(s)