Ehmt2^tm1.2Tara
Targeted Allele Detail

Summary

• Symbol: Ehmt2^tm1.2Tara
• Name: euchromatic histone lysine N-methyltransferase 2; targeted mutation 1.2, Alexander Tarakhovsky
• MGI ID: MGI:7380445
• Gene: Ehmt2 Location: Chr17:35117445-35133028 bp, + strand Genetic Position: Chr17, 18.45 cM
• Alliance: Ehmt2^tm1.2Tara page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:317372
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 23 and 24 were floxed and an FRT-flanked neo cassette inserted into intron 24 via homologous recombination. The selection cassette was then removed by flp mediated recombination. Cre-mediated recombination resulted in out-of-frame splicing and nonsense mediated decay of the mutant transcript. (J:317372)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ehmt2 Mutation: 57 strains or lines available

References

• Original: J:317372 Zylicz JJ, et al., G9a regulates temporal preimplantation developmental program and lineage segregation in blastocyst. Elife. 2018 May 10;7:e33361
• All: 1 reference(s)