Dnmt1^{tm1(CAG-Hras*G12V,-EGFP)Yfuj}
Targeted Allele Detail

Summary

• Symbol: Dnmt1^{tm1(CAG-Hras*G12V,-EGFP)Yfuj}
• Name: DNA methyltransferase 1; targeted mutation 1, Yasuyuki Fujita
• MGI ID: MGI:7378346
• Synonyms: DNMT1-CAG-loxP-STOP-loxP-HRas^V12-IRES-eGFP
• Gene: Dnmt1 Location: Chr9:20818501-20871084 bp, - strand Genetic Position: Chr9, 7.66 cM
• Alliance: Dnmt1^{tm1(CAG-Hras*G12V,-EGFP)Yfuj} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:249527
• Parent Cell Line: IB10/E14IB10 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: A targeting vector containing the CAG promoter, loxP-flanked STOP and neomycin resistance cassette, cDNA encoding Hras with a glycine to valine mutation at codon 12 (HRas^V12), FRT-flanked IRES-EGFP, and the diphtheria toxin subunit A gene was inserted into the locus. (J:249527)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnmt1 Mutation: 113 strains or lines available

References

• Original: J:249527 Kon S, et al., Cell competition with normal epithelial cells promotes apical extrusion of transformed cells through metabolic changes. Nat Cell Biol. 2017 May;19(5):530-541
• All: 5 reference(s)