Tg(Arc-tTA,-EGFP/Capzb*)10Mkyph
Transgene Detail

Summary

• Symbol: Tg(Arc-tTA,-EGFP/Capzb*)10Mkyph
• Name: transgene insertion 10, Maki Yamada
• MGI ID: MGI:7344056
• Synonyms: AiCE-Tg, C57BL/6N-Tg(Arc-tTA/egfp/capzb*2)100Mkyph
• Transgene: Tg(Arc-tTA,-EGFP/Capzb*)10Mkyph Location: unknown
• Alliance: Tg(Arc-tTA,-EGFP/Capzb*)10Mkyph page

Transgene origin

• Strain of Origin: C57BL/6N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence, Reporter, Transactivator)
• Mutation: Insertion
• Tg(Arc-tTA,-EGFP/Capzb*)10Mkyph expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Capzb (MGI:104652)	beta2 splice variant

• Mutation details: The transgenic construct contains sequence coding for a tetracycline-controlled transactivator (tTA), followed by a P2A self cleaving peptide, sequence coding for an EGFP/modified Capzb (capping actin protein of muscle Z-line subunit beta) fusion protein, and polyA signal sequence under the control of the 7 kb mouse Arc (activity regulated cytoskeletal-associated protein) promoter. EGFP-CapZ retains native C-terminus region that caps F-actin and binds other major binding proteins. The beta2 splice variant is expressed primarily in the brain and less so in striated muscle, which has Capz in the Z disc. (J:296392)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:296392 Kuboyama K, et al., Traceable stimulus-dependent rapid molecular changes in dendritic spines in the brain. Sci Rep. 2020 Sep 17;10(1):15266
• All: 2 reference(s)