Gt(ROSA)26Sor^{em1.1(CAG-Kcnq2*/EGFP)Hjch}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1.1(CAG-Kcnq2*/EGFP)Hjch}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1.1, Hee Jung Chung
• MGI ID: MGI:7341876
• Synonyms: cKcnq2^M547V
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1.1(CAG-Kcnq2*/EGFP)Hjch} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Inserted expressed sequence, Reporter)
• Mutations: Insertion, Nucleotide substitutions
• Gt(ROSA)26Sor^{em1.1(CAG-Kcnq2*/EGFP)Hjch} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Gt(ROSA)26Sor (MGI:104735)	M547V mutation

• Mutation details: A CAG-loxP-STOP-loxP-Kcnq2 (M547V; ATG to GTG) cDNA-IRES-EGFP-polyA cassette is introduced into intron 1 in reverse transcriptional orientation by CRISPR/cas9 methodologies. Cre-mediated recombination removed the STOP. (J:329150)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 956 strains or lines available

References

• Original: J:329150 Kim EC, et al., Spontaneous seizure and memory loss in mice expressing an epileptic encephalopathy variant in the calmodulin-binding domain of Kv7.2. Proc Natl Acad Sci U S A. 2021 Dec 21;118(51):e2021265118
• All: 1 reference(s)