Plk4^em4Ahol
Endonuclease-mediated Allele Detail

Summary

• Symbol: Plk4^em4Ahol
• Name: polo like kinase 4; endonuclease-mediated mutation 4, Andrew Holland
• MGI ID: MGI:7341824
• Synonyms: Plk4^cKD
• Gene: Plk4 Location: Chr3:40754463-40771318 bp, + strand Genetic Position: Chr3, 19.54 cM
• Alliance: Plk4^em4Ahol page

Mutation origin

• Strain of Origin: (C57BL/6 x SJL/J)F1

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: CRISPR/Cas9 genome editing uses guide RNAs, targeting DNA sequence 5'-aagctaggactttaatactc-3';, are used to insert a loxP flanked STOP cassette into intron 4 followed by a kinase-dead point mutation (D154A) into exon 5. Plk4-201 was used as reference for the exon number and the guide sequences. (J:329155)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Plk4 Mutation: 38 strains or lines available

References

• Original: J:329155 LoMastro GM, et al., PLK4 drives centriole amplification and apical surface area expansion in multiciliated cells. Elife. 2022 Aug 15;11:e80643
• All: 1 reference(s)