Trp53^tm6.2Glo
Targeted Allele Detail

Summary

• Symbol: Trp53^tm6.2Glo
• Name: transformation related protein 53; targeted mutation 6.2, Guillermina Lozano
• MGI ID: MGI:7341397
• Synonyms: Trp53^R245W
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm6.2Glo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:324785
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: An arginine to tryptophan (C-to-T change at position 733 and C-to-G change at position 735) substitution was introduced at codon 245 (p.R245W) in exon 7. In addition, a loxP site, Trp53 exons 7-11 with a native polyadenylation signal sequence, and FRT site flanked PGK neomycin resistance gene cassette and second loxP site were inserted in intron 6. The PGK neomycin cassette was removed via Flp-mediated recombination. The floxed exons 7-11 were removed via cre-mediated recombination in the germline resulting in the expression of the R245W mutation.

Tumor Data

List all tumor models in MMHCdb carrying Trp53^tm6.2Glo

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 248 strains or lines available

References

• Original: J:324785 Xiong S, et al., Differential Gain-of-Function Activity of Three p53 Hotspot Mutants In Vivo. Cancer Res. 2022 May 16;82(10):1926-1936
• All: 2 reference(s)