Trp53^tm5.1Glo
Targeted Allele Detail

Summary

• Symbol: Trp53^tm5.1Glo
• Name: transformation related protein 53; targeted mutation 5.1, Guillermina Lozano
• MGI ID: MGI:7341393
• Synonyms: Trp53^wm-R172H
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm5.1Glo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:268678
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Single point mutation
• Mutation details: An arginine to histidine substitution (G-to-A at position 515) was introduced at codon 172 (p.R172H) in exon 5. In addition, a loxP site, Trp53 exons 5-11 with a native polyadenylation signal sequence, and FRT site flanked PGK puromycin resistance gene cassette and second loxP site were inserted in intron 4. The PGK puromycin cassette was removed via Flp-mediated recombination. (J:268678)

Tumor Data

List all tumor models in MMHCdb carrying Trp53^tm5.1Glo

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 248 strains or lines available

References

• Original: J:268678 Zhang Y, et al., Somatic Trp53 mutations differentially drive breast cancer and evolution of metastases. Nat Commun. 2018 Sep 27;9(1):3953
• All: 7 reference(s)