Clcn3^tm3.1Tjj
Targeted Allele Detail

Summary

• Symbol: Clcn3^tm3.1Tjj
• Name: chloride channel, voltage-sensitive 3; targeted mutation 3.1, Thomas J Jentsch
• MGI ID: MGI:7339242
• Synonyms: Clcn3^ven
• Gene: Clcn3 Location: Chr8:61363423-61436334 bp, - strand Genetic Position: Chr8, 30.9 cM
• Alliance: Clcn3^tm3.1Tjj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:297238
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: A puromycin resistance cassette flanked by loxP sites was introduced between exon 1 and 2. Venus cDNA was inserted onto the start ATG of the open reading frame which codes for the transcript variant a. Additionally, a silent point mutation was introduced to obtain an NcoI restriction site. The Venus and Clcn3 sequences are separated by a 12 bp linker. The puromycin cassette was removed via cre-mediated recombination. (J:297238)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Clcn3 Mutation: 118 strains or lines available

References

• Original: J:297238 Weinert S, et al., Uncoupling endosomal CLC chloride/proton exchange causes severe neurodegeneration. EMBO J. 2020 May 4;39(9):e103358
• All: 1 reference(s)