Cnot6l^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cnot6l^{tm1c(EUCOMM)Wtsi}
• Name: CCR4-NOT transcription complex, subunit 6-like; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7338945
• Synonyms: Cnot6l^f
• Gene: Cnot6l Location: Chr5:96218192-96312030 bp, - strand Genetic Position: Chr5, 47.29 cM
• Alliance: Cnot6l^{tm1c(EUCOMM)Wtsi} page
• IMPC: Cnot6l gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:328670
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 96278689 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 96279613. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:328670)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cnot6l Mutation: 45 strains or lines available

References

• Original: J:328670 Katsumura S, et al., Deadenylase-dependent mRNA decay of GDF15 and FGF21 orchestrates food intake and energy expenditure. Cell Metab. 2022 Apr 5;34(4):564-580.e8
• All: 1 reference(s)