Esco2^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Esco2^{tm1d(EUCOMM)Wtsi}
• Name: establishment of sister chromatid cohesion N-acetyltransferase 2; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7338920
• Synonyms: Esco2 KO
• Gene: Esco2 Location: Chr14:66056476-66071418 bp, - strand Genetic Position: Chr14, 34.36 cM, cytoband D1
• Alliance: Esco2^{tm1d(EUCOMM)Wtsi} page
• IMPC: Esco2 gene page

Mutation origin

• Mutant Cell Line: EPD0409_6_A03
• Germline Transmission: Earliest citation of germline transmission: J:328654
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 66066877 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 66067838. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:328654)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Esco2 Mutation: 35 strains or lines available

References

• Original: J:328654 Wang J, et al., Reduced sister chromatid cohesion acts as a tumor penetrance modifier. PLoS Genet. 2022 Aug;18(8):e1010341
• All: 1 reference(s)