F2r^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: F2r^{tm1b(EUCOMM)Hmgu}
• Name: coagulation factor II thrombin receptor; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7335946
• Synonyms: PAR1^-
• Gene: F2r Location: Chr13:95738311-95754995 bp, - strand Genetic Position: Chr13, 50.21 cM
• Alliance: F2r^{tm1b(EUCOMM)Hmgu} page
• IMPC: F2r gene page

Mutation origin

• Mutant Cell Line: HEPD0904_4_C05
• Germline Transmission: Earliest citation of germline transmission: J:328121
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 95738027 of Chromosome 13 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 95741931. The critical exon(s) is/are thus flanked by loxP sites. Cre-mediated recombination deleted the critical exon(s) and generated a reporter knockout mouse. (J:328121)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any F2r Mutation: 23 strains or lines available

Notes

Cell line HEPD0904_4_C05 was used to create a reporter knockout allele which demonstrated germline transmission.

References

• Original: J:328121 Bochenek ML, et al., EPCR-PAR1 biased signaling regulates perfusion recovery and neovascularization in peripheral ischemia. JCI Insight. 2022 Jul 22;7(14):e157701
• All: 1 reference(s)