Lgals3bp^em2Lumc
Endonuclease-mediated Allele Detail

Summary

• Symbol: Lgals3bp^em2Lumc
• Name: lectin, galactoside-binding, soluble, 3 binding protein; endonuclease-mediated mutation 2, Leiden University Medical Centre
• MGI ID: MGI:7331630
• Synonyms: Lgals3bp-co, Lgals3bp-fl
• Gene: Lgals3bp Location: Chr11:118283573-118292787 bp, - strand Genetic Position: Chr11, 83.14 cM, cytoband E
• Alliance: Lgals3bp^em2Lumc page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: This allele was generated by a 3' loxP site insertion in intron 5 via electroporation of C57BL/6J zygotes of CRISPR/Cas9 RNP (sgRNA sequence TTGTGACCATTTGTCTGCAA; chr11:118,287,207-118,287,226 GRCm39/mm39) and a loxP-containing ssDNA oligonucleotide (200bp). Subsequent CRISPR/Cas9-targeting with RNPs (sgRNA sequence CAATGTCCCGTTGCTAAGTG - Chr11:118289852-118289871 GRCm39/mm39) and a ssDNA oligonucleotide (200bp) inserted the 5' loxP site in intron 2, to generate the final conditional mouse model. (J:329329)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lgals3bp Mutation: 32 strains or lines available

References

• Original: J:329329 Hohenstein P, Direct Data Submission Lgals3bp CRISPR alleles. MGI Direct Data Submission. 2021
• All: 1 reference(s)