Nsd3^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Nsd3^{tm1c(EUCOMM)Hmgu}
• Name: nuclear receptor binding SET domain protein 3; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7331557
• Synonyms: Nsd3^LoxP
• Gene: Nsd3 Location: Chr8:26091617-26209694 bp, + strand Genetic Position: Chr8, 14.17 cM
• Alliance: Nsd3^{tm1c(EUCOMM)Hmgu} page
• IMPC: Nsd3 gene page

Mutation origin

• Mutant Cell Line: HEPD0563_4_G02
• Germline Transmission: Earliest citation of germline transmission: J:302098
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 26139071 of Chromosome 8 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 26139966. The critical exon 4 is thus flanked by loxP sites. Flp-mediated recombination removed the lacZ and the neomycin resistance gene and generated an exon 4 floxed conditional allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:302098)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nsd3 Mutation: 67 strains or lines available

References

• Original: J:302098 Yuan G, et al., Elevated NSD3 histone methylation activity drives squamous cell lung cancer. Nature. 2021 Feb;590(7846):504-508
• All: 1 reference(s)