Tnks2^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tnks2^{tm1c(KOMP)Wtsi}
• Name: tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7329793
• Synonyms: Tnks2^flox
• Gene: Tnks2 Location: Chr19:36811632-36870877 bp, + strand Genetic Position: Chr19, 31.96 cM
• Alliance: Tnks2^{tm1c(KOMP)Wtsi} page
• IMPC: Tnks2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:327264
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 36828419 of Chromosome 19 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 36830538. The critical exon(s) is/are thus flanked by loxP sites.A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:327264)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tnks2 Mutation: 48 strains or lines available

References

• Original: J:327264 Matsumoto Y, et al., Tankyrase represses autoinflammation through the attenuation of TLR2 signaling. J Clin Invest. 2022 Apr 1;132(7):e140869
• All: 1 reference(s)