Map4^em1Yuhu
Endonuclease-mediated Allele Detail

Summary

• Symbol: Map4^em1Yuhu
• Name: microtubule-associated protein 4; endonuclease-mediated mutation 1, Yuesheng Huang
• MGI ID: MGI:7328685
• Synonyms: MAP4 KI
• Gene: Map4 Location: Chr9:109760528-109913023 bp, + strand Genetic Position: Chr9, 59.83 cM
• Alliance: Map4^em1Yuhu page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/Cas9 technology replaced exon 9 with exons containing a serine to alanine substitution at amino acid 667 (S667A), a serine to glutamate substitution at amino acid 737 (S737E), and a serine to glutamate substitution at amino acid 760 (S760E). This knock-in mimics MAP4 hyperphosphorylation at S737 and S760 and unphosphorylated S667 which is seen in tetralogy of Fallot patients. (J:278780)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Map4 Mutation: 230 strains or lines available

References

• Original: J:278780 Li L, et al., Microtubule associated protein 4 phosphorylation leads to pathological cardiac remodeling in mice. EBioMedicine. 2018 Nov;37:221-235
• All: 2 reference(s)