Mymx^em#Eno
Endonuclease-mediated Allele Detail

Summary

• Symbol: Mymx^em#Eno
• Name: myomixer, myoblast fusion factor; endonuclease-mediated mutation #, Eric N Olson
• MGI ID: MGI:7327601
• Synonyms: Myomixer KO
• Gene: Mymx Location: Chr17:45911897-45913028 bp, - strand Genetic Position: Chr17, 22.59 cM
• Alliance: Mymx^em#Eno page

Mutation origin

• Strain of Origin: (C57BL/6 x C3H)F1

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 genome editing technology was used to generate deletions in the gene. The cutting sites of the two sgRNAs were 122 base pairs apart in the third exon. PCR with primers amplifying this region showed mutant mice with deletions that ranged from 163 to 470 bp, reflecting different indels. The lines were not distinguished in the reporting of data in the original reference, and thus the nomenclature includes a # sign to indicate pooled data. (J:242061)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mymx Mutation: 27 strains or lines available

References

• Original: J:242061 Bi P, et al., Control of muscle formation by the fusogenic micropeptide myomixer. Science. 2017 Apr 21;356(6335):323-327
• All: 1 reference(s)