Gt(ROSA)26Sor^{tm13(Rr209-lacZ/GFP)Amc}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm13(Rr209-lacZ/GFP)Amc}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 13, Andrew P McMahon
• MGI ID: MGI:7327535
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm13(Rr209-lacZ/GFP)Amc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:327127
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: This allele was created in cells containing the Gt(ROSA)26Sor^{tm11(flpo)Amc} RMCE allele. The exchange vector used contains the following elements to replace the sequence between the F3 and FRT sites in the RMCE allele: an F3 site, a neomycin resistance gene cassette minus promoter and ATG translation start site, three poly(A) signal sequences, chicken beta-globin locus insulator sequence, Nkx2-2 enhancer Rr209 (450 bp XbaI-SalI fragment), the hsp68 promoter, a lacZ beta-galactosidase and nuclear GFP reporter fusion gene, poly(A) signal sequence and an FRT site. (J:327127)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

References

• Original: J:327127 Tsanov KM, et al., An embryonic stem cell-based system for rapid analysis of transcriptional enhancers. Genesis. 2012 May;50(5):443-50
• All: 1 reference(s)