Mirc53^tm1.2Oshea
Targeted Allele Detail

Summary

• Symbol: Mirc53^tm1.2Oshea
• Name: microRNA cluster 53, including Mir221 and Mir222; targeted mutation 1.2, John O'Shea
• MGI ID: MGI:7327298
• Synonyms: Mir221Mir222 KO, Mirc53^-
• Gene: Mirc53 Location: unknown Genetic Position: ChrX, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:305774
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Intergenic deletion
• Mutation details: A loxP site was inserted approximately 400 bp upstream from the start of Mir222 pre-miRNA. A neomycin cassette, second loxP site, and IRES-eGFP were inserted approximately 200 bp downstream from the end of Mir221 pre-miRNA. The neo cassette was excised via flpe-mediated recombination. Cre-mediated recombination deleted Mir221 and Mir222. (J:305774)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mirc53 Mutation: 1 strain or line available

References

• Original: J:305774 Mikami Y, et al., MicroRNA-221 and -222 modulate intestinal inflammatory Th17 cell response as negative feedback regulators downstream of interleukin-23. Immunity. 2021 Mar 9;54(3):514-525.e6
• All: 2 reference(s)