Mrps5^tm1.2Dshc
Targeted Allele Detail

Summary

• Symbol: Mrps5^tm1.2Dshc
• Name: mitochondrial ribosomal protein S5; targeted mutation 1.2, Dimitri Shcherbakov
• MGI ID: MGI:7327185
• Synonyms: MRPS5^G315R
• Gene: Mrps5 Location: Chr2:127429346-127445906 bp, + strand Genetic Position: Chr2, 62.05 cM, cytoband F3
• Alliance: Mrps5^tm1.2Dshc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:325948
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Insertion
• Mutation details: This allele was created using the FLEx switch system. A loxP site was inserted upstream of exon 10, a lox511 site, inverted exon 10 containing a glycine to arginine substitution at amino acid 315 (p.G315R), an FRT flanked neomycin selection cassette, loxP site, and lox511 site were inserted downstream of exon 10. The neomycin cassette was removed via flp-mediated recombination. Cre-mediated recombination in the germline removed the wild-type exon 10 and flipped the duplicated inverted exon bearing the G315R mutation resulting in expression of the mutant allele. (J:325948)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mrps5 Mutation: 43 strains or lines available

References

• Original: J:325948 Juskeviciene R, et al., Phenotype of Mrps5-Associated Phylogenetic Polymorphisms Is Intimately Linked to Mitoribosomal Misreading. Int J Mol Sci. 2022 Apr 15;23(8)
• All: 1 reference(s)