Gtf2i^tm1Ggcn
Targeted Allele Detail

Summary

• Symbol: Gtf2i^tm1Ggcn
• Name: general transcription factor II I; targeted mutation 1, Giuseppe Giaccone
• MGI ID: MGI:7327126
• Synonyms: Gtf2i L424H floxed KI
• Gene: Gtf2i Location: Chr5:134266688-134343614 bp, - strand Genetic Position: Chr5, 74.48 cM
• Alliance: Gtf2i^tm1Ggcn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:350001
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NCr

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The targeting vector is designed to insert a cassette containing floxed exons 15-34 and a mutated endogenous exon 15 downstream of the cassette. Specifically, the vector included, from 5' to 3', a loxP site, splice acceptor, cDNA encoding exons 15-34, a STOP cassette, a PGK-neo cassette, and a second loxP site downstream of endogenous exon 14. Downstream of the cassette, endogenous exon 15 was modified to harbor a Chr. 7: 74146970 T to A substitution, resulting in a leucine to histidine mutation (L424H), identified as the most frequent mutation in human thymic epithelial tumors (TETs). (J:101977, J:350001)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gtf2i Mutation: 137 strains or lines available

References

• Original: J:350001 He Y, et al., A Knock-In Mouse Model of Thymoma With the GTF2I L424H Mutation. J Thorac Oncol. 2022 Dec;17(12):1375-1386
• All: 2 reference(s)