Cdk5rap2^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cdk5rap2^{tm1b(EUCOMM)Wtsi}
• Name: CDK5 regulatory subunit associated protein 2; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:7325426
• Synonyms: Cdk5rap2^tm1b
• Gene: Cdk5rap2 Location: Chr4:70135092-70328672 bp, - strand Genetic Position: Chr4, 35.92 cM, cytoband C2
• Alliance: Cdk5rap2^{tm1b(EUCOMM)Wtsi} page
• IMPC: Cdk5rap2 gene page

Mutation origin

• Mutant Cell Line: EPD0381_4_F01
• Germline Transmission: Earliest citation of germline transmission: J:326669
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 70294808 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 70295589. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:326669)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdk5rap2 Mutation: 87 strains or lines available

References

• Original: J:326669 Tatrai P, et al., Centrosome function is critical during terminal erythroid differentiation. EMBO J. 2022 Jul 18;41(14):e108739
• All: 1 reference(s)