Nptn^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Nptn^{tm1c(EUCOMM)Hmgu}
• Name: neuroplastin; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7316907
• Synonyms: Nptn^fl
• Gene: Nptn Location: Chr9:58489504-58560162 bp, + strand Genetic Position: Chr9, 31.79 cM
• Alliance: Nptn^{tm1c(EUCOMM)Hmgu} page
• IMPC: Nptn gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:326721
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 58547403 of Chromosome 9 upstream of the critical exons 5 and 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the exon 6 at position 58551244. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:326721)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nptn Mutation: 39 strains or lines available

References

• Original: J:326721 Newton S, et al., Neuroplastin genetically interacts with Cadherin 23 and the encoded isoform Np55 is sufficient for cochlear hair cell function and hearing. PLoS Genet. 2022 Jan;18(1):e1009937
• All: 1 reference(s)