Plk4^em2Ahol
Endonuclease-mediated Allele Detail

Summary

• Symbol: Plk4^em2Ahol
• Name: polo like kinase 4; endonuclease-mediated mutation 2, Andrew Holland
• MGI ID: MGI:7316651
• Synonyms: Plk4^f
• Gene: Plk4 Location: Chr3:40754463-40771318 bp, + strand Genetic Position: Chr3, 19.54 cM
• Alliance: Plk4^em2Ahol page

Mutation origin

• Strain of Origin: (C57BL/6J x SJL/J)F1

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready)
• Mutation: Insertion
• Mutation details: CRISPR/cas9 genome editing uses two sgRNAs RNAs (mPLK4 sgRNA1: 5'-aagctaggactttaatactc-3', mPLK4 sgRNA2: 5'-ctgcatgtagagggaagctg-3') and ssDNA to introduce two loxP sites into intron 4 and 5. (J:329155)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Plk4 Mutation: 36 strains or lines available

References

• Original: J:329155 LoMastro GM, et al., PLK4 drives centriole amplification and apical surface area expansion in multiciliated cells. Elife. 2022 Aug 15;11:e80643
• All: 3 reference(s)