Col1a1^{tm1(tetO-Plk4,-EYFP)Ahol}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-Plk4,-EYFP)Ahol}
• Name: collagen, type I, alpha 1; targeted mutation 1, Andrew Holland
• MGI ID: MGI:7316646
• Synonyms: TetOn-Plk4
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-Plk4,-EYFP)Ahol} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:272389
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Col1a1^{tm1(tetO-Plk4,-EYFP)Ahol} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Plk4 (MGI:101783)

• Mutation details: A targeting vector is designed to insert FRT-flanked PGK-Neo-promoterless, ATG-less hygromycin cassette 500 bp downstream of the 3' UTR of the Col1a1 locus. Flpe recombinase is then used to FLP-IN a tetO-Plk4-EYFP plasmid. The Plk4-EYFP plasmid contained mouse Plk4 open reading frame (ORF) C-terminally tagged with EYFP downstream of the tetracycline response element (tetO) and CMV minimal promoter. (J:272389)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

References

• Original: J:272389 Levine MS, et al., Centrosome Amplification Is Sufficient to Promote Spontaneous Tumorigenesis in Mammals. Dev Cell. 2017 Feb 6;40(3):313-322.e5
• All: 2 reference(s)