Dot1l^em1Fiep
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dot1l^em1Fiep
• Name: DOT1 like histone lysine methyltransferase; endonuclease-mediated mutation 1, Patrick E Fields
• MGI ID: MGI:7316614
• Gene: Dot1l Location: Chr10:80591040-80631295 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Dot1l^em1Fiep page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:326664
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Endonuclease-mediated (Hypomorph)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/cas9 mediated recombination introduced an AA to GC mutation in exon 9 creating an asparagine 241 to alanine substitution. This mutation lies within the histone catalytic domain and eliminates H3K79 methylation activity. (J:326664)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dot1l Mutation: 75 strains or lines available

References

• Original: J:326664 Malcom CA, et al., Primitive Erythropoiesis in the Mouse is Independent of DOT1L Methyltransferase Activity. Front Cell Dev Biol. 2021;9:813503
• All: 1 reference(s)