Tg(NKX2-5*-lacZ)#Kyuhl
Transgene Detail

Summary

• Symbol: Tg(NKX2-5*-lacZ)#Kyuhl
• Name: transgene insertion, Kyu-Ho Lee
• MGI ID: MGI:7314938
• Synonyms: CNkx2.5-SHFnm-lacZ
• Transgene: Tg(NKX2-5*-lacZ)#Kyuhl Location: unknown Genetic Position: ChrUN, Syntenic
• Alliance: Tg(NKX2-5*-lacZ)#Kyuhl page

Transgene origin

• Strain of Origin: FVB

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: A mutated version of the chicken second heart field (SHF) NK2 homeobox 5 enhancer (cardiac activating region 3 (CAR3); nucleotides 976-3047 after transcriptional start site), where the Nkx2-5-binding consensus sequence in the BMP response element (BMPRE) was mutated from TCTGAGTG to TCTGCGAG, was used to drive the expression of a reporter gene cassette containing the hsp68 promoter, lacZ beta-galactosidase gene and poly(A) signal sequences by placing it downstream of the cassette. The transgene also contains a 5' HS4 insulator sequence from the chicken beta-globin locus. Founder information and line numbers were not available. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:192448)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:192448 Clark CD, et al., Evolutionary conservation of Nkx2.5 autoregulation in the second heart field. Dev Biol. 2013 Feb 1;374(1):198-209
• All: 1 reference(s)