Pafah1b1^tm2.1Awb
Targeted Allele Detail

Summary

• Symbol: Pafah1b1^tm2.1Awb
• Name: platelet-activating factor acetylhydrolase, isoform 1b, subunit 1; targeted mutation 2.1, Anthony Wynshaw-Boris
• MGI ID: MGI:7314834
• Gene: Pafah1b1 Location: Chr11:74564775-74615210 bp, - strand Genetic Position: Chr11, 45.76 cM, cytoband B3
• Alliance: Pafah1b1^tm2.1Awb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:326380
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A floxed PGK-neo cassette was inserted into intron 2 and a third loxP site was introduced into intron 6 via homologous recombination. The neomycin selection cassette was removed via flp-mediated recombination leaving exons 3-6 floxed. (J:326380)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pafah1b1 Mutation: 158 strains or lines available

References

• Original: J:326380 Penisson M, et al., Lis1 mutation prevents basal radial glia-like cell production in the mouse. Hum Mol Genet. 2022 Mar 21;31(6):942-957
• All: 1 reference(s)