Csn1s1^{em1(CSF2)Nrba}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Csn1s1^{em1(CSF2)Nrba}
• Name: casein alpha s1; endonuclease-mediated mutation 1, Nariman R Battulin
• MGI ID: MGI:7312048
• Synonyms: INS-GM
• Gene: Csn1s1 Location: Chr5:87814067-87830437 bp, + strand Genetic Position: Chr5, 43.56 cM
• Alliance: Csn1s1^{em1(CSF2)Nrba} page

Mutation origin

• Strain of Origin: 129S2/SvPasCrl

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Csn1s1^{em1(CSF2)Nrba} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CSF2 (1437)

  Csf2	(MGI:1339752)

• Mutation details: CRISPR/Cas9 technology inserted the human CSF2 open reading frame behind the mouse endogenous Csn1s1 start codon, preserving the remaining Csn1s1 sequence. (J:326408)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Csn1s1 Mutation: 31 strains or lines available

Notes

DGES1 ES cells (129S2/SvPasCrl) were used.

References

• Original: J:326408 Smirnov AV, et al., Evaluation of the alpha-casein (CSN1S1) locus as a potential target for a site-specific transgene integration. Sci Rep. 2022 May 14;12(1):7983
• All: 1 reference(s)